基于SiO2荧光纳米粒子快速检测果汁中 大肠杆菌的免疫传感器研究
目的 设计一种基于SiO2荧光纳米粒子快速检测果汁中大肠杆菌O157:H7的新型传感器。方法 采用超分子组合法将荧光基团嵌入到SiO2纳米颗粒中合成SiO2荧光纳米粒子, 然后将抗大肠杆菌O157:H7的单抗偶联到纳米粒子表面, 最后通过抗原-抗体反应使纳米粒子与待检细菌结合, 使用荧光显微镜观察并统计大肠杆菌O157:H7的个数。结果 本方法与革兰氏染色法相比, 对大肠杆菌O157:H7计数结果无统计学差异(P=0.930); 该方法整个检测过程能够在15 min内完成, 检测下限小于10 CFU/mL。结论 该方法能增强检测果汁中大肠杆菌O157:H7的灵敏度, 缩短检测时间, 对其他病原体检测方法的改进具有重要的指导意义。
Objective To design a new biosensor for rapid detection of Escherichia coli O157:H7 in juice by synthesized luminophore-doped silica (LDS) nanoparticles. Methods The fluorescent groups were embed into SiO2 nanoparticles to synthesize LDS by supramolecular combination method. Then E. coli O157:H7 specific monoclonal antibody was coupled to the surface of the dye doped nanoparticles. Finally, antibody-coated narnoparticles incorporated with the cells of O157:H7 by antigen-antibody reaction to form conglutinations, which represented the images of the detected germs and could be observed and counted by fluorescence microscope. Results The number of E. coli O157:H7 was detected and counted with methods of fluorescent nanoparticles staining and gram staining, which had no significant difference (P=0.930). The novel developed LDS nanoparticles could finish the detection within 15 mins, and had the limit of detection of 10 CFU/mL. Conclusion The method of LDS nanoparticles can enhance the sentivity of O157:H7 detection and obviously shorten testing time, which has important significance for the improvement of inspection methods for other pathogenic microorganisms.
标题：基于SiO2荧光纳米粒子快速检测果汁中 大肠杆菌的免疫传感器研究
英文标题：Biosensor for rapid detection of Escherichia coli in juice by luminophore-doped silica
作者：
孙晓萌 山东省医学科学院基础医学研究所
张志涛 山东省医学科学院基础医学研究所
王轶鹏 山东省医学科学院基础医学研究所
许强 山东省医学科学院基础医学研究所
孙雪梅 山东省医学科学院基础医学研究所
刘文敏 山东省医学科学院基础医学研究所
中文关键词:SiO2荧光纳米粒子,大肠杆菌O157:H7,快速检测,生物传感器,果汁,
英文关键词:luminophore-doped silica,Escherichia coli O157:H7,rapid detection,biosensor,juice,
发表日期：2017-03-16
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