DNA条形码在肉制品掺伪中非定向筛查技术的研究
目的 对基于DNA条形码的肉制品掺伪中非定向筛选技术进行探索。方法 以线粒体基因组中COI基因为靶标, 设计猪、牛、羊、马、鸡、鸭、鹅、鼠8种动物源物种间的通用引物和特异性引物, 建立禽畜肉的DNA条形码检测方法。同时应用该技术对50份市售的肉制品进行检测。结果 本研究建立了DNA条形码筛选技术, 电泳条带通过基因测序能正确识别猪、牛、羊、马、鸡、鸭、鹅、鼠8种动物源成分, 结合分子克隆技术能实现对混合肉的检测。50份市售肉制品的DNA条形码结果显示, 16份掺杂了除牛羊肉以外的其他禽畜肉。结论 DNA条形码技术能打破传统标准中PCR法检测目标唯一性的局限, 具有良好的应用前景。
Objective To research non-directional test method of meat adulteration based on DNA barcode. Methods Based on COI gene in mitochondria, universal primers and specific primers of pork, beef, mutton, horsemeat, chicken, duck, goose and rat were designed and DNA barcode method was established. And then 50 meat products bought from markets were detected by this method. Results Pork, beef, mutton, horsemeat, chicken, duck, goose and rat meat could be distinguished with each other after sequencing gene of electrophoresis banding in the established DNA barcode method. Combined with molecular cloning technique, mixed meat could be tested by this method. The DNA barcode test results of 50 meat products showed that 16 of them were adulterated with other livestock meat except for beef and mutton. Conclusion DNA barcode can break the limitation of which the target of PCR method is unique in traditional standard, and this technology has the extremely broad application prospect.
标题：DNA条形码在肉制品掺伪中非定向筛查技术的研究
英文标题：Research on the non-directional test in meat adulteration based on DNA barcode
作者：
钟文涛 国家农副产品质量监督检验中心,湖南省产商品质量监督检验研究院,湖南师范大学生命科学院
王芳妹 国家农副产品质量监督检验中心,湖南省产商品质量监督检验研究院,湖南师范大学生命科学院
李白玉 国家农副产品质量监督检验中心,湖南省产商品质量监督检验研究院
蒋伟 国家农副产品质量监督检验中心,湖南省产商品质量监督检验研究院
颜亨梅 湖南师范大学生命科学院
中文关键词:DNA条形码,COI基因,肉制品掺伪,非定向筛查,
英文关键词:DNA barcode,COI,meat adulteration,non-directional detection,
发表日期：2017-05-08
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