花生过敏原Ara h7基因的克隆及序列分析
目的 克隆花生Ara h7基因cDNA序列, 并对其序列进行分析。方法 采用Trizol法, 从花生子叶中提取花生RNA, 经反转录PCR, 克隆花生Ara h7 cDNA序列; 采用生物信息学软件对Ara h7序列进行分析, 预测蛋白结构及功能。结果 Ara h7 cDNA序列有482 bp, 编码160个氨基酸。生物信息学分析结果显示Ara h7蛋白是一种亲水性蛋白, 分子量为18.881 kDa, 具有9个潜在的磷酸化位点, 5个不同的B细胞线性表位。结论 本研究成功克隆了Ara h7 cDNA序列, 并采用生物信息学软件分析了基因编码的蛋白的特征。
Objective To clone and analyze the Ara h7 cDNA sequences. Methods Total RNA was extracted from peanuts cotyledon by Trizol reagents. Ara h7 cDNA sequences were cloned through RT-PCR. The sequences were analyzed and protein properties were predicted using bioinformatics tools. Results The length of cDNA of Ara h7 was 482 bp and it coded 160 amino acids. The bioinformatical analysis showed that Ara h7 was a 18.881 kDa hydrophilic protein with 9 potential phosphorylation sites and 5 different B cell linear epitopes. Conclusion This study cloned the cDNA of Ara h7 successfully and analyzed the coded amino acid properties of Ara h7 with bioinformatics tools.
标题：花生过敏原Ara h7基因的克隆及序列分析
英文标题：Cloning and sequence analysis of peanut allergy Ara h7 gene
作者：
周新虹 上海睿智化学研究有限公司
中文关键词:过敏原,花生,生物信息学,反转录PCR,Ara h7,
英文关键词:allergy,peanuts,bioinformatics,reverse transcription PCR,Ara h7,
发表日期：2017-01-15
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