热加工对乳清中主要过敏原潜在致敏性的影响
目的 探讨不同温度条件下, 热加工对牛乳中主要过敏原潜在致敏性的影响。方法 将α-乳白蛋白与β-乳球蛋白经不同的加热条件处理后, 用间接ELISA检测上述2种过敏原蛋白IgG的结合能力的变化。结果 热处理后的α-乳白蛋白的IgG结合能力呈上升趋势; 但经60~75 ℃热处理的α-乳白蛋白均比未加工的抗原性低, 经60 ℃热处理的α-乳白蛋白的IgG结合能力下降幅度最大, 下降比例为40%; 经80 ℃热处理的α-乳白蛋白IgG结合能力大于未处理的α-乳白蛋白。热处理对β-乳球蛋白的IgG结合能力的影响与α-乳白蛋白相反: 在60~80 ℃热加工条件下, β-乳球蛋白的抗原性随着温度的升高, 抗原性逐渐减小, 且经80 ℃加热处理的β-乳球蛋白的IgG结合能力最低, 但仍然大于未加工的β-乳球蛋白的IgG结合能力。结论 热加工能改变α-乳白蛋白和β-乳球蛋白IgG结合能力, 进而改变致敏性。
Objective To investigate the effect of thermal processing on the potential allergenicity of major allergens in milk under different temperature conditions. Methods The binding ability of α-lactalbumin and β-lactoglobulin after treated with different heating conditions to IgG was detected by ELISA. Results The results showed that the binding ability of α-lactalbumin to IgG after thermal processing was increased, but the binding ability of α-lactalbumin treated at 60~75 ℃ was lower than that of untreated antigen. The binding ability of α-lactalbumin to IgG after treated at 60 ℃ was the largest while decreased by 40% compared with untreated antigen. The binding ability of α-lactalbumin to IgG after treated at 80 ℃ was bigger than untreated α-lactalbumin. It was opposite that the effect of thermal processing on the binding ability of β-lactoglobulin to IgG compared with α-lactalbumin: with the increase of temperature, the antigenicity of β-lactoglobulin decreased at 60~80 ℃, while the binding ability of β-lactoglobulin after treated at 80 ℃ was the lowest but still bigger than that of untreated β-lactoglobulin. Conclusion Thermal processing can change the binding ability of α-lactalbumin and β-lactoglobulin to IgG, and then change the sensitization.
标题：热加工对乳清中主要过敏原潜在致敏性的影响
英文标题：Effects of thermal processing on the potential allergenicity of major allergens in whey
作者：
费爽雯 南昌大学食品科学与技术国家重点实验室
白浩 南昌大学食品科学与技术国家重点实验室
李欣 南昌大学食品科学与技术国家重点实验室
曾弘华 江西省丰城市剑光医院
陈红兵 南昌大学食品科学与技术国家重点实验室,南昌大学中德研究院
李守明 江西省儿童医院
中文关键词:热加工,牛乳过敏原,α-乳白蛋白,β-乳球蛋白,
英文关键词:thermal processing,milk allergens,α- lactalbumin,β-lactoglobulin,
发表日期：2017-01-15
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