实时定量荧光PCR快速鉴定食品中单核细胞增生李斯特氏菌
目的 建立实时定量荧光PCR法(real-time PCR)快速鉴定食品中的单核细胞增生李斯特氏菌(Listeria monocytogenes, LM)。方法 选取2016年国家食品风险监测样本134例与模拟灭活LM样本10例, 采用GB/T4789.30-2010与real-time PCR方法同步检测单核细胞增生李斯特菌。结果 共检测食品134份, 包括肉制品、水产品、快餐和即食食品等。共检出8株LM, 检出率为5.97%。以GB/T4789.30-2010为金标准判断, real-time PCR方法检测样本中LM的灵敏度与特异度均达到100%。模拟灭活LM样本real-time PCR方法检出率为100%, 标准法检出率为0%。结论 本方法可以简化实验程序, 减少工作量, 节约检测试剂, 为可能发生的食物中毒尽早提供实验依据。
Objective To establish the method for rapid identification of Listeria monocytogenes (LM) in food by real-time PCR. Methods One hundred and thirty-four cases of national food risk monitoring samples in 2016 and 10 cases of simulated inactivated LM samples were simultaneously detected by GB/T4789.30-2010 method and real-time PCR method. Results A total of 134 samples of food including meat products, aquatic products, fast food and instant food etc. were enrolled. Totally 8 strains of LM were detected and the detection rate was 5.97 %. Using GB/T4789.30-2010 as the gold standard, the sensitivity and specificity of real-time PCR were both up to 100%. In 10 samples of simulated inactivated LM, the detection rate of real-time PCR method was 100%, but that of the standard method was 0%. Conclusion The established real-time PCR method can simplify the experiment procedure, reduce the workload and save the reagent, which can provide experimental basis for probable food poisoning as early as possible.
标题：实时定量荧光PCR快速鉴定食品中单核细胞增生李斯特氏菌
英文标题：Rapid detection of Listeria monocytogenes in food by real-time PCR
作者：
刘万静 西安交通大学第一附属医院检验科
刘斌 安康市疾病预防控制中心生物检验科
李湘平 安康市疾病预防控制中心生物检验科
李海燕 安康市疾病预防控制中心生物检验科
石红 西安交通大学第一附属医院检验科
吴友伟 西安交通大学第一附属医院检验科
答嵘 西安交通大学第一附属医院检验科
中文关键词:单核细胞增生李斯特氏菌,实时定量荧光PCR,食品,
英文关键词:Listeria monocytogenes,real-time PCR,food,
发表日期：2016-10-13
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