猪源性动物产品中猪链球菌2型分离方法的建立和应用
目的 建立猪源性动物产品中猪链球菌2型分离方法, 为监测人畜共患致病菌猪链球菌2型提供技术支持。方法 通过选择不同增菌基础液、不同种类和不同浓度的抑菌剂及不同选择性分离平板, 建立猪源性动物产品中猪链球菌2型的分离方法。对从920份猪源性动物产品分离到的可疑菌落进行初步筛选、生化鉴定、血清学鉴定和分子鉴定。结果 使用该分离方法检测猪源性动物产品, 背景细菌显著减少, 猪链球菌典型菌落显著增加, 分离更加容易。从920份猪源性动物产品中共检出27株猪链球菌, 阳性率为2.93%(27/920), 其中猪链球菌2型为17株, 阳性率为1.85%(17/920)。结论 本方法为猪源性动物产品中猪链球菌2型检测提供了技术手段。
Objective To establish isolation methods for detection of Streptococcus suis type 2, so as to provide technical support for monitoring zoonotic pathogen in pig-derived products. Methods By choosing different enrichment base fluid, different concentrations of different kinds of bacteriostatic agent and different selective separating plates, a method for isolation of Streptococcus suis serotype 2 in pig-derived animal products was established. Preliminary screening, biochemical identification, serological identification and molecular identification of suspicious colonies isolated from 920 pig-derived products were carried out. Results It was easier to isolate Streptococcus suis type 2 in pig-derived products, where the background bacteria were significantly reduced, and the typical colonies increased significantly. A total of 27 strains of Streptococcus suis were detected from 920 pig-derived products, with the positive rate of 2.93% (27/920), and 17 strains were Streptococcus suis type 2, with positive rate of 1.85% (17/920). Conclusion The established method is suitable for detection of Streptococcus suis type 2 in pig-derived products.
标题：猪源性动物产品中猪链球菌2型分离方法的建立和应用
英文标题：Establishment and application of isolation methods for Streptococcus suis type 2 in pig-derived products
作者：
陈国强 苏州出入境检验检疫局,江苏出入境检验检疫局,广东省动植物与食品进出口技术措施研究重点实验室
袁飞 中国检验检疫科学研究院
王凯民 江苏出入境检验检疫局
陈雷 江苏出入境检验检疫局
祝长青 江苏出入境检验检疫局
陈培根 苏州出入境检验检疫局
张睿 江苏出入境检验检疫局
张常印 江苏出入境检验检疫局
中文关键词:猪源性动物产品,猪链球菌2型,分离,
英文关键词:pig-derived products,Streptococcus suis type 2,isolation,
发表日期：2017-08-31
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