云南文山鲜三七花、茎、叶、根中人参皂甙成分测定

云南文山鲜三七花、茎、叶、根中人参皂甙成分测定
目的  建立鲜三七花、茎、叶、根中7种人参皂甙成分分析的超高效液相色谱测定方法, 并测定云南文山产鲜三七花、茎、叶、根中7种人参皂甙成分。方法  样品粉碎后用水超声提取, 提取液离心, 取上清液通过Amberlite-XAD大孔树脂, 用甲醇洗脱后蒸干, 再用初始流动相定容, 上机分析。采用二极管阵列检测器(diode array detector, DAD)检测, 外标法定量。结果  在14 min内完成7种目标化合物的分离分析, 人参皂甙Rg1、Re、Rb1、Rc、Rb2、Rb3和Rd分别在0.0240~0.6005、0.0198~0.4960、0.0272~0.6810、0.0222~0.5545、0.0199~0.4974、0.0202~0.5055和0.0216~0.5395 μg范围内呈良好的线性关系, 各组分回收率范围为78.5%~95.4%, RSD为0.55%~0.77%(n=6)。结论  该方法灵敏、快速、准确, 可用于云南文山产鲜三七花、茎、叶、根中人参皂甙成分的检测。

Objective  To establish a method for the determination of ginsenosides of different parts of fresh Panax Notoginsengs by ultra performance liquid chromatography (UPLC), and determine the content of 7 kinds of ginsenosides in flowers, stems, leaves and roots of fresh Panax Notoginsengs from Wenshan, Yunnan province. Methods  Sample were pulverized with water ultrasonic extraction, the extraction liquid was centrifuged, the cleaning liquid was removed by Amberlite-XAD large hole resin and then evaporated with methanol, then the initial flow phase was determined and analyzed by the instrument. The diode array detector and the external standard method were used. Results  The separation of 7 target compounds had finished within 14 minutes. The 7 kinds of ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3 and Rd had good linear relationships in the ranges of 0.0240~0.6005, 0.0198~0.4960, 0.0272~0.6810, 0.0222~0.5545, 0.0199~0.4974, 0.0202~0.5055, and 0.0216~0.5395 μg, respectively. The recovery ranges of the components were 78.5%~95.4%, with the relative standard deviations (RSDs) of 0.55%~0.77% (n=6). Conclusion  The method is sensitive, rapid and accurate, which can be used in the detection of ginsenosides in flowers, stems, leaves and roots of fresh Panax Notoginsengs from Wenshan, Yunnan province.

标题：云南文山鲜三七花、茎、叶、根中人参皂甙成分测定
英文标题：Determination of ginsenosides in flower, stem, leaf and root of fresh Panax Notoginseng from Wenshan, Yunnan province

作者：
刘阳 云南省疾病预防控制中心
段志敏 云南省疾病预防控制中心
熊宏苑 云南省疾病预防控制中心
许燕 云南省疾病预防控制中心
徐丹先 云南省疾病预防控制中心
林佶 云南省疾病预防控制中心

中文关键词:三七,超高效液相色谱,人参皂甙,
英文关键词:Panax Notoginsengs,ultra performance liquid chromatography,ginsenosides,

发表日期：2017-05-11

2025-1-30 19:38 上传 云南文山鲜三七花、茎、叶、根中人参皂甙成分测定.pdf

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