毛细管电泳-电化学发光法检测鳀鱼中氧氟沙星
目的 针对水产品中氧氟沙星的快速分离分析, 建立以Ru(bpy)32+为发光源的毛细管电泳-电化学发光体系, 并将其应用于鳀鱼中氧氟沙星含量的检测。方法 针对氧氟沙星的分离检测对各项条件进行优化, 并且在最优条件下对氧氟沙星标准品进行检测。结果 得到其线性方程为I=134.27c+46.32, 线性相关系数r=0.9981(n=5), 检出限(S/N=3)为0.08 μg/mL。使用该方法, 对新鲜鳀鱼样品中的氧氟沙星含量进行分离检测, 结合检测结果通过计算得到峰高及迁移时间的相对标准偏差(RSD)值分别为0.26%、1.08%, 样品中氧氟沙星含量为0.287 μg/mL。结论 该方法具有分离效率高、灵敏度高、样品消耗低、成本低等优点, 适用于水产品内氧氟沙星的快速分离检测。
Objective To establish a method for determination the content of ofloxacin in anchovy by capillary electrophoresis-electrochemiluminescence (CE-ECL) system based on Ru(bpy)32+, for the rapid separation and analysis of ofloxacin in aquatic products. Methods The separation and detection of ofloxacin was optimized, and the standard of ofloxacin was tested under optimal conditions. Results Using CE-ECL, under the optimum conditions for the detection of ofloxacin, the linear equation was I=134.27c+46.32, while the linear correlation coefficient was 0.9981 (n=5), with detection limit (S/N=3) of 0.08 μg/mL. Using this method for the determination of ofloxacin content in anchovy samples, the relative standard deviations (RSDs) of high peak and migration time were 0.26% and 1.08%. The content of ofloxacin in the sample was 0.287 μg/mL. Conclusion The method has the advantages of high separation efficiency, high sensitivity, low sample consumption and low cost, which is suitable for the detection of ofloxacin in aquatic products.
标题：毛细管电泳-电化学发光法检测鳀鱼中氧氟沙星
英文标题：Determination of ofloxacin content in anchovy by capillary electrophoresis-electrochemiluminescence
作者：
陈思远 集美大学食品与生物工程学院
陈竹秋 集美大学诚毅学院信息工程系
王力 集美大学诚毅学院信息工程系
中文关键词:毛细管电泳-电化学发光联用法,三联吡啶钌,氧氟沙星,
英文关键词:capillary electrophoresis-electrochemiluminescence,Rucbpy,ofloxacin,
发表日期：2017-07-11
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