微芯片技术在农产品及食品真菌毒素快速检测中的应用
真菌毒素污染的农产品及食品会对人类及牲畜的健康产生严重威胁。目前, 真菌毒素的检测主要采用液相色谱-质谱联用法(liquid chromatography-mass spectrometry, LC-MS)、气相色谱-质谱联用法(gas chromatography-mass spectrometry, GC-MS)等传统检测方法。然而, 这些检测方法的样品前处理过程比较繁琐、费时, 并且在分析过程中还会消耗大量的有毒试剂。微芯片技术所需样品的消耗量少并且分析时间短, 可实现样品的集成化、微型化以及高通量检测。微芯片技术在真菌毒素检测中的应用, 弥补了上述传统检测方法的不足。本文主要综述了微芯片技术在农产品及食品真菌毒素快速检测中的应用研究进展。首先对农产品及食品中常见的真菌毒素及其毒性进行了简单介绍; 接着重点对微芯片技术在真菌毒素检测中的应用进行了详细论述; 最后对微芯片应用于真菌毒素检测的发展前景和挑战进行了展望。
Mycotoxin contamination in agricultural and food products has threatened the health of human and animals. Conventional methods for the detection of mycotoxins primarily include the liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). However, all these methods require extensive sample preparation procedures and are time consuming. Moreover, large amounts of hazardous regents and solvents are often used during analysis. Microchip requires less sample consumption and short analysis time, and can realize the integration, miniaturization and high-throughput detection of samples. The application of microchip in the detection of mycotoxins can overcome the deficiency of the above traditional detection methods. This review focused on the application of microchip in detection of mycotoxins in agricultural products and food samples. The mycotoxins and their toxicity in agricultural products and food samples were firstly summarized. Then the application of microchip in detection of mycotoxins were reviewed in detail. Finally, challenges and future research directions in the development of microchip in detection of mycotoxins were previewed.
标题：微芯片技术在农产品及食品真菌毒素快速检测中的应用
英文标题：Application of microchip in the detection of mycotoxins in agricultural and food products
作者：
满燕 北京市农林科学院,北京农业质量标准与检测技术研究中心,农业部农产品质量安全风险评估实验室(北京),农产品产地环境监测北京市重点实验室
梁刚 北京市农林科学院,北京农业质量标准与检测技术研究中心,农业部农产品质量安全风险评估实验室(北京),农产品产地环境监测北京市重点实验室
李安 北京市农林科学院,北京农业质量标准与检测技术研究中心,农业部农产品质量安全风险评估实验室(北京),农产品产地环境监测北京市重点实验室
靳欣欣 北京市农林科学院,北京农业质量标准与检测技术研究中心,农业部农产品质量安全风险评估实验室(北京),农产品产地环境监测北京市重点实验室
潘立刚 北京市农林科学院,北京农业质量标准与检测技术研究中心,农业部农产品质量安全风险评估实验室(北京),农产品产地环境监测北京市重点实验室
中文关键词:真菌毒素,微流控芯片,检测,,
英文关键词:mycotoxin, microchip, detection,
发表日期：2017-06-01
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