基于微间隙阵列电极传感器检测副溶血性弧菌的方法初探
目的 建立电化学免疫传感器法快速检测副溶血性弧菌的分析方法。方法 采用微间隙阵列电极作为基础电极, 运用双抗夹心酶联免疫反应和酶催化银沉积反应实现分析信号放大, 检测副溶血性弧菌。结果 该方法特异性良好, 对非目标菌株无交叉反应, 对副溶血性弧菌的检出限为105 CFU/mL, 线性范围为105~108 CFU/mL, 传感器的电导率与副溶血性弧菌浓度之间呈良好的线性关系, 线性相关系数r2=0.955。增菌6 h后, 只需2 h即可完成检测。结论 该方法具有检测速度快、操作简便、检测成本低等特点, 为副溶血性弧菌的快速检测提供了一种有效手段。
Objective To establish a method for rapid determination of Vibrio parahaemolyticus by microgapped electrode array sensor. Methods Vibrio parahaemolyticus was detected using a microgapped electrode array sensor combined with biological enzyme catalyzed silver deposition reaction to amplify the analysis signal. Results This method only had a specific reaction to Vibrio parahaemolyticus, and no cross reaction to other strains. The detection limit of Vibrio parahaemolyticus was 105 CFU/mL. In the concentration range of 105~108 CFU/mL, there was a good linear relationship between the conductivity of the sensor and the concentration of Vibrio parahaemolyticus, and the linear correlation coefficient (r2) was 0.955. It could be completed within 2 h after enrichment of 6 h. Conclusion This method has characteristics of rapid, simplicity and low cost, which provides an effective method for rapid detection of Vibrio parahaemolyticus.
标题：基于微间隙阵列电极传感器检测副溶血性弧菌的方法初探
英文标题：Primary research on detection of Vibrio parahaemolyticus using microgapped electrode array sensor
作者：
杨丽霞 长沙市食品药品检验所
邱华丽 长沙市食品药品检验所
周金沙 长沙市食品药品检验所
邱志鹏 长沙市食品药品检验所
中文关键词:微间隙阵列电极传感器,副溶血性弧菌,酶催化银沉淀,
英文关键词:microgapped electrode array sensor,Vibrio parahaemolyticus,enzymatic silver deposition,
发表日期：2017-04-24
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