液相色谱四极杆飞行时间质谱法测定 橙汁中的桔霉素
目的 建立快速测定橙汁中桔霉素的液相色谱/四极杆飞行时间质谱方法。方法 采用QuEChERS法提取净化样品, 外标法定量, 液相色谱/四极杆飞行时间质谱法检测。结果 桔霉素在0.005~0.2 mg/L浓度范围内有良好的线性(r>0.9997)。在3个不同添加水平下, 样品的加标回收率范围为82.62%~104.13%, 相对标准偏差(RSD)<3%。将本方法用于实际样品分析, 20份市售橙汁产品中有4份检测到桔霉素。结论 QuEChERS结合液相色谱/四极杆飞行时间质谱检测方法简便快速, 可用于测定橙汁中的桔霉素。
Objective To establish a method for determination of citrinin in orange juice by liquid chro-matography-hybrid quadrupole time-of-flight mass spectrometry (LC-Q-TOF). Methods The samples were extracted by QuEChERS method, and citrinin were separated by LC-Q-TOF, then measured by external standard method. Results Citrinin standard curve had a good linearity relationship within 0.005~0.2 mg/L (r>0.9997). Average recovery rate of samples was between 82.62% and 104.13% at 3 adding levels, and RSD<3.0%. Totally 20 orange juice samples by the current method was successfully performed. The citrinin was observed in 4 samples. Conclusion The quantitative method is rapid, simple, and high sensitive, which can be applied in the accurate determination of citrinin in orange juice.
标题：液相色谱四极杆飞行时间质谱法测定 橙汁中的桔霉素
英文标题：Determination of citrinin in orange juice by liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry
作者：
李艳波 福建农业职业技术学院生物技术系
陈晓波 福建农业职业技术学院生物技术系
史怀 福建省农业科学院农业生物资源研究所
中文关键词:桔霉素,橙汁,液相色谱/四极杆飞行时间质谱,QuEChERS,
英文关键词:citrinin,orange juice,liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry,QuEChERS,
发表日期：2015-11-20
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