微膜富集原位表面增强拉曼法测定蔬果中 3种残留杀菌剂
目的 建立微膜富集原位表面增强拉曼光谱法快速检测蔬果中3种杀菌剂的残留量。方法 采用过滤法将银(金)纳米粒子直接负载于有机尼龙66微孔滤膜上, 该微孔滤膜分离富集蔬果中福美双、噻菌灵和啶酰菌胺3种杀菌剂后, 采用表面增强拉曼光谱法进行原位检测。结果 方法的线性范围分别为0.010~1.0、0.10~20和0.10~5.0 mg/L, 检出限分别为0.50、39 和5.8 μg/L。该方法应用于苹果、梨、橙、柚子、玉米、土豆和黄瓜等蔬果中的杀菌剂残留量分析, 回收率在88.0%~110%之间, 相对标准偏差均小于10%。结论 该方法简单、快速、准确、灵敏, 适用于蔬果等食品中杀菌剂类农药残留的分析检测。
Objective To establish a rapid method combined with micromembrane enrichment and surface-enhanced Raman spectroscopy for the detection of pesticides in vegetables and fruits. Methods Ag/Au nanoparticles were adsorbed onto the organic nylon 66 microporous filter membrane through a filtration process. When thiram, thiabendazole and boscalid in vegetables and fruits were successfully enriched with fabricated micromembrane substrates, in-situ detected by surface enhanced Raman spectroscopy was followed. Results The linear ranges of the present method for thiram, thiabendazole and boscalid were 0.010~1.0, 0.10~20 and 0.10~5.0 mg/L and their detection limits were 0.50, 39 and 5.8 μg/L, respectively. While applied in the analysis of thiram, thiabendazole and boscalid in apple, pear, corn, grapefruit, potato, orange and cucumber, the recoveries were ranged of 88.0%~110%, and the relative standard deviations were less than 10%. Conclusion The present method is simple, rapid, sensitive and accurate, which is suitable for the detection of residue pesticides in different foods.
标题：微膜富集原位表面增强拉曼法测定蔬果中 3种残留杀菌剂
英文标题：Micromembrane enrichment and in-situ surface enhanced Raman spectroscopy for the detection of pesticides in vegetables and fruits
作者：
陈小曼 中山大学化学与化学工程学院
陈漾 中山大学化学与化学工程学院
李攻科 中山大学化学与化学工程学院
胡玉玲 中山大学化学与化学工程学院
肖小华 中山大学化学与化学工程学院
中文关键词:微膜富集,表面增强拉曼光谱,原位检测,农药残留,蔬菜,水果,
英文关键词:micromembrane enrichment,surface-enhanced Raman spectroscopy,in-situ,pesticides,vegetables,fruits,
发表日期：2015-10-23
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