呋喃妥因代谢物CLIA试剂盒对畜禽类及水产品验证研究
目的 验证呋喃妥因代谢物CLIA检测试剂盒的检测效果。方法 用化学发光微粒子免疫法和高效液相色谱-串联质谱法分别对市售猪肉、鸡肉、鱼肉、虾肉4种样品中呋喃妥因代谢物残留量进行检测, 比对两种方法试验结果间的差异。结果 该试剂盒对4种样品的最低检测限分别为 69.37、67.86、67.52、69.09 ng/kg, 试剂盒板内板间变异系数均小于10%; 对4种样品做加标回收试验, 其回收率均在 90%~105%之间, 变异系数均小于10%; 该方法与高效液相色谱法检测实际样品的阴、阳性判断结果一致。结论 该方法稳定、可靠, 可满足食品中呋喃妥因代谢物残留快速检测的需要。
Objective To verify the test effect of chemiluminescence immune (CLIA) kit. Methods The method of CLIA was used to detect the residues of furantoin metabolites in 4 kinds of samples including market sold pork, chicken, fish and shrimp, and the results were compared with that of high performance liquid chro-matography-tandem mass spectrometry. Results The minimum detection limitation of the kit of the 4 kinds of samples were 69.37, 67.86, 67.52, and 69.09 ng/kg, respectively, and the coefficient of variation was less than 10%.The recovery of the method was in the range of 90%~105%, and the coefficient of variation was less than 10%. The CLIA's judgments of positive and negative were the same as the HPLC's. Conclusion This method is efficient, reliable and sensitive, and can meet the fast-tested need for the determination of furantoin metabolites residues in food.
标题：呋喃妥因代谢物CLIA试剂盒对畜禽类及水产品验证研究
英文标题：Verification of furantoin metabolites CLIA kits in meat, poultry and seafood
作者：
谢体波 贵州勤邦食品安全科学技术有限公司
党娟 贵州勤邦食品安全科学技术有限公司
易重任 贵州勤邦食品安全科学技术有限公司
李平 贵州勤邦食品安全科学技术有限公司
何方洋 贵州勤邦食品安全科学技术有限公司
冯才伟 贵州勤邦食品安全科学技术有限公司
中文关键词:呋喃妥因代谢物,化学发光微粒子免疫法,高效液相色谱-串联质谱法,
英文关键词:furantoin metabolites,chemiluminescence immune method,high performance liquid chromatography tandem mass spectrometry,
发表日期：2015-10-21
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