利用反相阳离子交换混合模式色谱同时检测10种β-内酰胺类抗生素
建立了反相/弱阳离子交换混合模式色谱(C18/WCX)对10种β-内酰胺类抗生素(头孢羟氨苄、头孢替唑钠、头孢呋辛钠、头孢唑啉、头孢拉定、头孢西酮、头孢孟多酯钠、头孢噻吩、哌拉西林、氯唑西林钠)同时检测的方法。以乙腈/甲酸铵/水为流动相,与常规反相色谱分析Unitary C18为对照,结果表明,中性条件下优化流动相洗脱梯度,10种β-内酰胺类抗生素在C18/WCX具有较高的分离选择性。通过缓冲盐浓度和pH值的调控,发现C18/WCX与样品间分离机制由反相作用力与离子交换两种作用力共同作用。研究表明,混合模式色谱在β-内酰胺类抗生素的分离分析中具有较大的潜力。
Reversed-phase liquid chromatography/weak cation exchange (RPLC/WCX, C18/WCX) was established for the simultaneous determination of ten β-lactam antibiotics (Cefadroxil, Ceftezole sodium, Cefuroxime sodium, Cefazolin, Cephradine, Cefazedone, Cemandil sodium, Cephalothin, Pipracil and Cloxacillin sodium). A chromatographic separation was achieved on a C18/WCX with the mobile phase consisting of acetonitrile and ammonium formate mixture in a gradient mode. Compared with the conventional reversed-phase chromatography Unitary C18, the selectivity of C18/WCX can be improved. It was found that the mechanism of samples separated on C18/WCX were hydrophobic interaction and ion exchange interaction by regulated by the salt concentration and pH value. The results showed that the mixed-mode chromatography would be a potent approach for the analysis and separation of β-lactam antibiotics.
标题：利用反相阳离子交换混合模式色谱同时检测10种β-内酰胺类抗生素
英文标题：Application of reverse-phase cation exchange mixed-mode chromatography detection for the simultaneous determination of β-lactam antibiotics
作者：
刘名扬 大连交通大学环境与化学工程学院
郭祯 大连交通大学环境与化学工程学院
赵景红 辽宁出入境检验检疫局
朱程云 大连交通大学环境与化学工程学院;辽宁出入境检验检疫局;中国科学院大连化学物理研究所,分离分析化学重点实验室
刘月 大连交通大学环境与化学工程学院
中文关键词:混合模式色谱,反相/弱阳离子交换混合模式,β-内酰胺类抗生素,
英文关键词:mixed-mode chromatograpy,reversed-phase liquid chromatography/ weak cation exchange (RPLC/WCX),β-lactam antibiotics,
发表日期：2015-07-30
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