《保健食品检验与评价技术规范》中肉碱含量测定方法适用性的探讨

《保健食品检验与评价技术规范》中肉碱含量测定方法适用性的探讨
目的：对《保健食品检验与评价技术规范》（2003年版）肉碱含量测定的高效液相色谱法进行适用性研究。方法：通过对检测波长、色谱柱、流动相比例等色谱条件，提取剂、提取温度及提取时间等提取条件的试验，探讨了不同提取及色谱条件对检验结果的影响。结果：以纯化水为提取剂、30℃超声20 min，采用色谱柱Phenomenex Gemini C18，流动相 pH2.5 0.05mol/L磷酸氢二钾:0.002mol/L辛烷磺酸钠:乙腈（52:45:3），检测波长210nm，0.8mL/min，肉碱浓度在47.86 μg/mL～2.39 mg/mL范内呈良好的线性关系，(r=1.0000)，平均回收率为101.58%，RSD%值为1.46。结论：简化了提取剂配制步骤，优化了流动相的比例，改善了色谱柱的专属性，该试验条件适用于不同产品中左旋肉碱含量的测定，可提高肉碱质量标准的实验操作性。

[ ]　Objective: Studies on the HPLC method of the Content determination of L-carnitine in the weight reducing health food, and the influence on the experiment results by different chromatographic conditions and extraction conditions were investigated. Methods: The samples were extracted by ultrasonic extraction with pure water , the temperature was 30℃, and the time was 20 minutes. The separarion was performed on a Phenomenex Gemini C18,column ( 4. 6 ×150 mm, 5μm) , the mobile phase consisted of Hydrogen phosphate two(pH2.5 0.005mol/L)+Octane sulfonate(0.002mol/L) +Acetonitrile = 52:45:3 , the flow was 0.8 mL/min,the detection wavelength was 210 nm. Results: The  -camitine was shown to be good linear over the range from 47.86μg/ml～2.39mg/ml ( r = 1.0000). The Average recovery was 101.5% ,RSD 1.46%. Conclusion: The experiment conditions are suitable for the determination of L-carnitine in different products and can be used for the establishment of quality standards.

标题：《保健食品检验与评价技术规范》中肉碱含量测定方法适用性的探讨
英文标题：Discussion on the Standard applicability of the content of L-carnitine in The Technical Specification for Inspection and Evaluation of Health Food

作者：
潘小红 湖南省食品药品检验研究院
李涛 湖南省食品药品检验研究院
杨庆懿 湖南省食品药品检验研究院
林海 湖南省食品药品检验研究院

中文关键词:肉碱,高效液相色谱法,减肥类保健食品,《保健食品检验与评价技术规范》,
英文关键词:L-camitine,HPLC,Weight reducing health food,The Technical Specification for Inspection and Evaluation of Health Food,

发表日期：2015-06-15

2025-1-29 20:01 上传 《保健食品检验与评价技术规范》中肉碱含量测定方法适用性的探讨.pdf

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