QuEChERS净化超高效液相色谱法快速测定 豆制品中的9种工业染料
目的 建立了豆皮、豆腐、腐竹及豆干4种豆制品中的9种工业染料的检测方法。方法 以QuEChERS方法作为前处理技术, 以超高效液相色谱技术作为检测技术, 对方法的有效性进行了验证, 并对实际样品进行检测。结果 以空白的4种豆制品为基质, 分别在3个水平进行加标实验, 回收率分别在71.5%~126.4%、74.0%~106.5%、86%~124.5%和76.7%~107.5%之间。RSD (n=5) 分别在1.9%~8.5%、1.8%~8.1%、1.8%~7.0%之间和1.7%~8.0%之间。各工业染料的检出限(S/N=3)在0.05~0.2 mg/kg之间, 定量限(S/N=10)在0.15~0.6 mg/kg之间。结论 该方法操作简单, 稳定性好, 适于在实际检测工作中推广应用。
Objective To establish a method for the detection of 9 kinds of industrial dyes in Yuba, tofu, beancurd sticks,and dried tofu . Methods The validity of the method was validated, which used to the actual sample, with QuEChERS as a pretreatment technology and ultra-high performance liquid chromatography as detection method. Results Blank bean products material were used as matrixes, and spiking experiments were carried out in high, medium and low level, respectively. Recoveries were between 71.5% to 126.4%, 74.0% to 106.5%, 86% to 124.5% and 76.7%~107.5%. RSD (n=5) were between 1.9% to 8.5%, 1.8% to 8.1%, 1.8% to 7.0% and 1.7% to 8.0%, the limits of detection (S/N=3) were between 0.05~0.2 mg/kg and the limit of quantification (S/N=10) was between 0.15~0.6 mg/kg. Conclusion The method is simple to operate with a good stability and is suitable for the application in daily testing work.
标题：QuEChERS净化超高效液相色谱法快速测定 豆制品中的9种工业染料
英文标题：Determination of 9 kinds of dyes in bean products by ultra performance liquid chromatography with QuEChERS cleanup
作者：
路杨 河北省疾病预防控制中心河北省食品安全风险监测重点实验室
吕志强 廊坊市中医院
刘印平 河北省疾病预防控制中心河北省食品安全风险监测重点实验室
王丽英 河北省疾病预防控制中心河北省食品安全风险监测重点实验室
常凤启 河北省疾病预防控制中心河北省食品安全风险监测重点实验室
董彬 河北省疾病预防控制中心河北省食品安全风险监测重点实验室
杨立新 河北省疾病预防控制中心河北省食品安全风险监测重点实验室;北京大学环境科学与工程学院
中文关键词:工业染料,QuEChERS,超高效液相色谱法,豆制品,快速检测,
英文关键词:dye,QuECHERS,ultra performance liquid chromatography,bean products,rapid detection,
发表日期：2015-08-10
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