山东临沂部分县区花生收获前后黄曲霉毒素B1污染情况研究﹡
目的 了解花生从收获前到收获、储存期间黄曲霉毒素B1(AFB1)污染的环节，为预防花生AFB1的污染提供科学依据。方法 2014年，在山东临沂市选择花生种植范围较广的5个县，分别于收获前1～2周、收获时、收获晾干后1个月和储存3个月采集花生样本共260份，酶联免疫法(ELISA)检测花生AFB1的污染情况，并对结果进行分析。结果 260份花生样品中，收获前、收获时样品中AFB1均小于2 μg/kg，收获晾干后1个月和储存3个月各有1份样品AFB1含量严重超标，分别是81.07 μg/kg和为254.27 μg/kg，阳性率均为1.27%；总阳性率为0.77%。结论 2014年临沂部分地区花生中AFB1阳性率较低，污染主要发生在收获后的晾晒和储存期，提示科学晾晒和储存是预防花生AFB1污染的重要措施之一。
Objective To investigate aflatoxin B1 (AFB1) contamination of peanuts from pre-harvest, harvest period to storage period, and to provide a scientific basis to prevent contamination of AFB1 in peanuts. Methods In 2014, five counties of Linyi in Shandong province were chosen, and 260 peanut samples were collected in 1~2 weeks before harvest, harvest, post-harvest 1 month and 3 months storage, standardized enzyme-linked immunosorbent assay (ELISA) kits were used to detect AFB1 content in peanuts, then, the results were analyzed. Results In 260 peanut samples, AFB1 concentrations from all pre-harvest and harvest peanuts were less than 2 μg/kg, AFB1 of 2 samples from 1 month after drying and 3 months storage were out of limits, the values were 81.07 μg/kg and 254.27 μg/kg, respectively, the positive rate was 1.27%, and total positive rate was 0.77%. Conclusion AFB1 contamination of peanuts in this region occurred mostly in drying and storage period after harvest, scientific drying and storage were one of the most important measures to prevent AFB1 contamination in peanuts.
标题：山东临沂部分县区花生收获前后黄曲霉毒素B1污染情况研究﹡
英文标题：Contamination of aflatoxin B1 in peanuts around harvest from some counties of Linyi in Shandong Province
作者：
苏祥 山东大学公共卫生学院
刘建伟 山东大学公共卫生学院卫生微生物学检验系
韩小敏 国家食品安全风险评估中心
秦泽明 山东大学公共卫生学院卫生微生物学检验系
温红玲 山东大学公共卫生学院卫生微生物学检验系
宋艳艳 山东大学公共卫生学院卫生微生物学检验系
李凤琴 国家食品安全风险评估中心
赵丽 山东大学公共卫生学院卫生微生物学检验系
中文关键词:花生,黄曲霉毒素B1,酶联免疫法,污染调查,
英文关键词:peanuts,AFB1,ELISA,contamination survey,
发表日期：2015-07-24
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