氨基酸自动分析仪测定游离L-半胱氨酸
目的 对比讨论含硫氨基酸的传统测定方法过甲酸氧化法, 针对添加了L-半胱氨酸的保健食品, 提出一个操作简便、计算准确的测定游离L-半胱氨酸含量的方法。方法 称取试样用上机缓冲液超声溶解, 微孔滤膜过滤, 应用氨基酸自动分析仪分析, 用Na型离子交换色谱柱分离, L-半胱氨酸标准品定量计算其含量。结果 L-半胱氨酸浓度在0.3861~3.0648 mg/mL的范围内与峰面积的线性良好, 相关系数r>0.999。在80%、100%、120%添加水平下, L-半胱氨酸的平均回收率为97.2%。结论 该方法操作简便、准确、重现性好, 适用于保健食品中游离L-半胱氨酸的含量测定。
Objective By comparison of the traditional detection method for sulfur-containing amino acids, formic acid oxidation method, this paper puts forward a more simple operation method with accurate calculation of free L-cysteine content for those health food adding L-cysteine. Methods The sample was dissolved with buffer ultrasonic, filtered by microporous membrane filter, and analyzed by automatic amino acid analyzer. Na type ion exchange chromatography column separation was used. The content was calculated by L-cysteine standard quantitative calculation. Results The concentration of L-cysteine in the range of 0.3861~3.0648 mg/mL had a good linearity with peak area, the correlation coefficient r was >0.999. At the level of adding 80%, 100%, and 120%, the average recovery of L-cysteine was 97.2%. Conclusion The method is simple, accurate, reproducible and applicable for the determination of L-cysteine in health food.
标题：氨基酸自动分析仪测定游离L-半胱氨酸
英文标题：Determination of free L-cysteine by automatic amino acid analyzer
作者：
叶少文 汤臣倍健股份有限公司
中文关键词:含硫氨基酸,氨基酸自动分析仪,L-半胱氨酸,
英文关键词:sulfur-containing amino acid,automatic amino acid analyzer,L-cysteine,
发表日期：2015-06-09
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