双波长反相高效液相色谱法同时测定葛根姜黄 胶原蛋白片中葛根素、芍药苷和姜黄素
目的 建立一种同时测定葛根姜黄胶原蛋白片中葛根素、芍药苷和姜黄素的反相高效液相色谱法。方法 样品经50%乙醇超声提取, 经SUPELCO-C18柱分离, 流动相为甲醇-水, 采用梯度洗脱, 在0~25 min之间波长为230 nm, 在25.01~39 min之间波长为430 nm条件下检测。结果 葛根素、芍药苷和姜黄素在上述色谱条件下能完全分离。葛根素、芍药苷和姜黄素的线性范围为0.01~0.1 mg/mL; 平均回收率96.3~103.3%, RSD<2.0%。结论 该方法操作简便, 精密度和回收率高, 重复性好, 可同时测定葛根姜黄胶原蛋白片中葛根素、芍药苷和姜黄素。
Objective To establish a method for the determination of puerarin, paeoniflorin and curcumin in radix puerariae turmeric collagen tablets by reversed-phase high performance liquid chromatography. Methods Samples were ultrasonically extracted with 50% ethanol. The extracts were separated by SUPELCO-C18 column with gradient elution and methanol-water as the mobile phase, then samples were detected between 0~25 min at a wavelength of 230 nm and 25.01~39 min at a wavelength of 430 nm. Results Puerarin, paeoniflorin and curcumin could be completely separated by the conditions. The linear range was 0.01~0.1 mg/mL, and the average recovery was 93.5%~103.8%, RSD<2.0%.Conclusion The method is simple, and has a high precision, recovery rate and good repeatability. It can simultaneously determine puerarin, paeoniflorin and curcumin in radix puerariae turmeric collagen tablets.
标题：双波长反相高效液相色谱法同时测定葛根姜黄 胶原蛋白片中葛根素、芍药苷和姜黄素
英文标题：Determination of puerarin, paeoniflorin and curcumin in radix puerariae turmeric collagen tablets by reversed-phase high performance liquid chro-matography with dual-wavelength
作者：
苏昭仑 汤臣倍健股份有限公司
黄康惠 汤臣倍健股份有限公司
张喜金 汤臣倍健股份有限公司
陈彩云 汤臣倍健股份有限公司
陈宏壁 汤臣倍健股份有限公司
林贤邦 汤臣倍健股份有限公司
中文关键词:葛根素,芍药苷,姜黄素,反相高效液相色谱法,葛根姜黄胶原蛋白片,
英文关键词:puerarin,paeoniflorin,curcumin,reversed-phase high performance liquid chromatography,radix puerariae turmeric collagen tablets,
发表日期：2015-06-12
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