液相色谱法测定保健食品中硫酸软骨素的含量
目的 建立一种快速、简便测定保健食品中硫酸软骨素的液相色谱方法, 并进行方法学研究。方法 样品经过前处理, 以乙腈和0.005 mol/L戊烷磺酸钠溶液为流动相, 等度洗脱, 经Diamonsil C18柱分离, 于192 nm波长检测。结果 用本方法进行检测, 能很好地分离硫酸软骨素, 在浓度0.008 mg/mL~0.04 mg/mL之间呈现良好的线性关系, 方法的相关系数可达1.000, 平均回收率在98.5%~100.0%之间, 相对标准偏差为0.6%。结论 采用该方法能测定保健食品中硫酸软骨素, 耗时短、操作简便、准确、重现性好, 分离效果明显, 可在实验室推广。
Objective To investigate a quick and simple method for determination of chondroitin sulfate in health foods by high performance liquid chromatography (HPLC). Methods Samples were treated using 0.005 mol/L pentane solution of sodium-acetonitrile as mobile phase with isocratic elution, separated on diamonsil C18 column, and detected at 192 nm wavelength. Results Chondroitin sulfate could be separated well by this method. It had a good linear relationship within 0.008~0.04 mg/mL (r=1.000), the average recoveries was 98.5%~100.0%, and the RSD was 0.6%. Conclusion The method can be used for determination of chondroitin sulfate in health foods, it is quick, simple and accurate, and has a good reproducibility and clear separation, which can be popularized in the laboratory.
标题：液相色谱法测定保健食品中硫酸软骨素的含量
英文标题：Determination of chondroitin sulfate in health food by high performance liquid chromatography
作者：
陈彩云 汤臣倍健股份有限公司
蔡伟江 汤臣倍健股份有限公司
中文关键词:高效液相色谱法,保健食品,硫酸软骨素,含量测定,
英文关键词:high performance liquid chromatography,health foods,chondroitin sulfate,determination,
发表日期：2015-04-10
- 文件大小:
- 595.44 KB
- 下载次数:
- 60
-
高速下载
|
|
