人参茸芝胶囊的质量控制研究
目的 建立人参茸芝胶囊中人参、灵芝孢子粉的定性鉴别和标志性成分人参皂苷和灵芝酸A含量测定的方法。方法 用薄层色谱法(thin layer chromatography, TLC)定性鉴别人参茸芝胶囊中人参、灵芝孢子粉; 用高效液相色谱法(high performance liquid chromatography, HPLC)测定人参皂苷和灵芝酸A的含量。结果 薄层鉴别荧光斑点清晰, 分离度好, 阴性对照无干扰; 人参皂苷在0.5~50 μg范围内呈良好的线性关系, 平均回收率在98.53%~102.53%之间, 相对标准偏差(RSD)在0.84%~1.83%之间; 灵芝酸A的线性范围0.0006~0.06 mg/mL(r2=0.9998), 平均回收率为98.80%, RSD为0.89%。结论 所建立的人参、灵芝孢子粉定性鉴别、标志性成分人参皂苷和灵芝酸A定量检测方法可用于人参茸芝胶囊的质量控制。
Objective To establish the methods to qualitatively identify the ginseng and Ganoderma lucidum spore powder, and determine the ginsenosides and ganoderic acid A in the Renshen Rongzhi capsules. Methods Qualitative identification of ginseng and ganoderma spore in Renshen Rongzhi capsules was carried out by thin layer chromatography (TLC). Ginsenosides and Ganoderma acid A were determined by high per-formance liquid chromatography (HPLC). Results The TLC spots were clear with high resolution, and the blank test showed no interference. The linear range of ginsenosides was 0.5~50 μg, the average recovery was between 98.53%~102.53%, and the relative standard deviation (RSD) was between 0.84%~1.83%. The linear range of ganoderma acid A was 0.0006~0.06 mg/mL (r2=0.9998), the average recovery was 98.80%, and the RSD was 0.89%. Conclusion The qualitative and quantitative analysis methods are applicable for the quality control of Renshen Rongzhi capsules.
标题：人参茸芝胶囊的质量控制研究
英文标题：tudy on quality control for Renshen Rongzhi capsules
作者：
赵岩 吉林农业大学中药材学院
妙志岩 吉林农业大学中药材学院
牛淑静 吉林省食品药品审评中心
郜玉钢 吉林农业大学中药材学院
蔡恩博 吉林农业大学中药材学院
杨鹤 吉林农业大学中药材学院
刘双利 吉林农业大学中药材学院
张连学 吉林农业大学中药材学院
中文关键词:人参茸芝胶囊,薄层色谱法,高效液相色谱法,质量控制,
英文关键词:Renshen Rongzhi capsules,thin layer chromatography,high performance liquid chromatography,quality control,
发表日期：2015-01-15
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