多重荧光PCR鉴别羊肉掺假
目的 建立可同时检测掺入羊肉中的猪、马、牛、鸭成分的多重荧光PCR检测体系。方法 配制掺入猪、马、牛、鸭源性成分的模拟羊肉掺假样品, 掺假比例17%~45%。筛选特异性强、敏感度高的猪、马、牛、鸭物种特异性基因, 优化反应体系, 建立了针对猪、牛、马、鸭两两配对的两重荧光PCR检测体系和其中3个任意组合的三重荧光PCR检测体系。结果 模拟羊肉掺假样品两重荧光PCR检测结果显示, 包括单个成分掺入比例低至7%的样品, 其检测准确率为100%。而三重荧光PCR检测同时检测猪、牛、马、鸭四种肉品中的3种, 其中猪肉、牛肉和马肉的掺假比例均为8%~15%; 鸭牛肉掺假比例为5%~10%。三重荧光PCR检测结果显示, 所有掺假样品中的各种掺假组分都被准确检出。结论 本研究建立的多重荧光PCR检测体系能准确检出配制的模拟掺假羊肉制品中的猪、马、牛、鸭成分, 可进一步研究以应用于市场肉制品及加工肉制品掺假的检测。
Objective To establish multiplex fluorescent polymerase chain reaction (PCR) system, and simultaneously detect pig, horse, cattle and duck meat mixed in mutton. Methods Mutton samples were mixed with 17%~45% pig, horse, cattle and duck meat. The specific species genes were selected with good specificity and sensitivity to form random duplex and triplex fluorescent PCR detection system for pig, cattle, horse and duck. Results The duplex fluorescent PCR system could identify single added component as low as 7% for 100% accuracy. The triplex fluorescent PCR system could identify 8%~15% pig，cattle, horse and 5%~10% duck in any combination for 100% accuracy. Conclusion The established multiplex fluorescent PCR system can detect pig, horse, cattle and duck meat mixed in prepared mutton samples accurately. It can be further researched to application in market meat and processed meat adulteration detection.
标题：多重荧光PCR鉴别羊肉掺假
英文标题：Identification of mutton adulteration by multiplex fluorescent PCR
作者：
杨冬燕 深圳市疾病预防控制中心
韦梅霞 桂林医学院
杨永存 深圳市疾病预防控制中心
李浩 深圳市疾病预防控制中心
邓平建 深圳市疾病预防控制中心
中文关键词:羊肉,掺假,多重荧光PCR,
英文关键词:mutton,adulteration,multiplex fluorescent PCR,
发表日期：2014-11-28
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