响应面法优化亚临界水提取槟榔籽中槟榔碱的 工艺研究
目的 利用响应面试验优化亚临界水提取槟榔籽中槟榔碱的工艺。方法 在单因素实验的基础上, 利用单因素试验结合Box-Benhnken实验和响应面分析法, 研究提取温度、提取时间以及液料比3个因子对槟榔籽槟榔碱提取率的影响, 通过回归分析模拟得到二次多项式回归方程的预测模型。结果 亚临界水提取槟榔籽中槟榔碱的最佳工艺条件为提取温度144 ℃, 提取时间43 min, 液料比35:1(mL/g)。由模型预测提取率为4.23 mg/g, 验证实验结果为4.25 mg/g, 误差仅为0.47%, 与预测值较接近, 表明模型具有较好预测性。且各因子对提取率的影响大小依次是提取温度>提取时间>液料比。结论 亚临界水提取可以作为一种有效的槟榔籽中槟榔碱提取方法。
Objective To investigate the optimization of subcritical water extraction process of arecoline from areca seed (AAS) by response surface methodology. Methods The single-factor tests and response surface methodology were used to investigate the effect of three parameters (extraction temperature, extraction time and liquid-solid ratio) on the yield of AAS. A second- order polynomial regression model was established using a Box-Behnken experimental design. Results The optimum process conditions were as follows: extraction time of 43 min, extraction temperature of 143 ℃, liquid-to-solid ratio of 35: 1(mL/g), and the extraction rate of AAS was up to 4.25 mg/g, which was highly consistent with the predictive value of 4.23 mg/g, with the fractional error of 0.47%. It showed that the established regression equation for arecoline yield exhibited an excellent goodness of fit, and the orders of factors influencing the extraction of AAS were as follows: extraction temperature>extraction time>liquid-to-solid ratio. Conclusion Subcritical water extraction can be applied for the extraction of AAS.
标题：响应面法优化亚临界水提取槟榔籽中槟榔碱的 工艺研究
英文标题：Optimization of subcritical water extraction process of arecoline from areca seeds by response surface methodology
作者：
康丽茹 海南大学食品学院
符素芳 海南大学食品学院
曾广琳 海南大学食品学院
姬彦羽 海南大学食品学院
张海德 海南大学食品学院
张伟敏 海南大学食品学院
中文关键词:槟榔籽,槟榔碱,亚临界水提取,响应面,
英文关键词:areca seeds,arecoline,subcritical water extraction,response surface methodology,
发表日期：2016-07-18
- 文件大小:
- 1.42 MB
- 下载次数:
- 60
-
高速下载
|
|
