气相色谱法分析不同产地辣木叶中植物甾醇的含量
目的 研究西双版纳、韶关、安哥拉、印度和云南辣木叶中菜油甾醇、β谷甾醇和豆甾醇的含量差异。方法 样品经50%氢氧化钾皂化后, 用乙醚:正己烷(1:1,V:V)萃取游离甾醇并将溶剂蒸干, 用N,N-二甲基甲酰胺:六甲基二硅氨烷:三甲基氯硅烷(8:2:1,V:V:V)将游离甾醇硅烷化, 然后进行气相色谱分析, 并分析各地辣木叶中菜油甾醇、豆甾醇和β谷甾醇含量。结果 β谷甾醇在韶关辣木叶中含量为20 mg/100 g左右, 在西双版纳辣木叶中含量为40 mg/100 g,在安哥拉、印度和云南辣木叶中含量为30 mg/100 g左右; 菜油甾醇在韶关辣木叶中含量为5~7 mg/100 g, 在印度辣木叶中含量为8 mg/100 g,在西双版纳、安哥拉和云南辣木叶中均大于10 mg/100 g;豆甾醇在各个辣木叶中含量为2~12 mg/100 g。结论 西双版纳、云南、安哥拉、印度辣木叶中菜油甾醇、β谷甾醇含量明显高于韶关辣木叶, 豆甾醇含量没有明显的地域差异。
Objective To study the content difference of campesterol, β-sitosterin, and stigmasterol in Moringa oleifera leaves from Xishuangbanna, Shaoguan, Angola, India and Yunnan. Methods After saponification by 50% potassium hydroxide, the free phytosterol of samples were extracted by diethyl ether:hexane(1:1,V:V), and the solvent were evaporated to dryness before silanization using N,N-Dimethylformamide(DMF), hexamethyldisilazane (HMDS) and trimethyl-chlorosilane (TMCS)(8:2:1,V:V:V). The content of campesterol, stigmasterol and β-sitosterin in Moringa oleifera leaves from various areas were analyzed by gas chromatography (GC). Results The content of β-sitosterin were around 20 mg/100 g in Moringa oleifera leaves from Shaoguan, 40 mg/100 g in that from Xishuangbanna and 30 mg/100 g in those from Angola, India and Yunnan. The content of campesterol were around 5~7 mg/100 g in Moringa oleifera leaves from Shaoguan, 8 mg/100 g in that from India and larger than 10 mg/100 g in those from Xishuangbanna, Angola and Yunnan. The content of stigmasterol were 2~12 mg/100 g in Moringa oleifera leaves from different areas. Conclusion The content of campesterol and β-sitosterin of Moringa oleifera leaves from Xishuangbanna, Yunnan, Angola and India are remarkably higher than those from Shaoguan. The content of stigmasterol has no obvious regional differences.
标题：气相色谱法分析不同产地辣木叶中植物甾醇的含量
英文标题：Content determination of phytosterol in Moringa oleifera leaves from different areas by gas chromatography
作者：
杨扬 杭州娃哈哈集团有限公司,浙江省食品生物工程重点实验室
赵志红 杭州娃哈哈集团有限公司,浙江省食品生物工程重点实验室
李小佳 杭州娃哈哈集团有限公司,浙江省食品生物工程重点实验室
朱慧 杭州娃哈哈集团有限公司,浙江省食品生物工程重点实验室
中文关键词:气相色谱,辣木叶,植物甾醇,
英文关键词:gas chromatography,Moringa oleifera leaves,phytosterol,
发表日期：2016-07-01
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