新型C18核壳结构色谱柱快速分析人参中的 3种皂苷
目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定人参中人参皂苷Rg1、Re和Rb1 3种人参皂苷的含量。方法 采用月旭BoltimateTM C18新型核壳色谱柱对3种人参皂苷进行分离, 以乙腈-1.0%磷酸水溶液为流动相进行梯度洗脱, 采用HPLC进行检测。结果 3种人参皂苷在月旭BoltimateTM C18新型核壳色谱柱能够快速分离, 且分离效果较好。3种人参皂苷的在0.4~4.0 μg质量浓度范围内呈良好的线性关系, 线性相关系数(R2)分别为0.9998, 0.9996和0.9997; Rg1、Re和Rb1检出限分别为0.003、0.001和0.002 μg/kg; 加标回收率为98.3%~101.3%, 相对标准偏差RSD分别为0.8%、1.1%和1.5%(n=6)。结论 本方法具有快速、柱压低及节省溶剂等优势, 可用于人参提取物中人参皂苷的测定。
Objective To establish a method for the determination of 3 kinds of ginsenosides including Rg1, Re and Rb1 in Panax ginseng by high performance liquid chromatography (HPLC). Methods Samples were separated by Welch BoltimateTM C18 core-shell column with the mobile phase of acetonitrile and phosphoric acid aqueous solution for gradient elution, and then detected by HPLC. Results Using Welch BoltimateTM C18 core-shell column, 3 kinds of ginsenosides could be separated rapidly and well. The calibration curves showed good linear relationships in the mass concentration range of 0.4~4.0 μg with correlation coefficients of 0.9998, 0.9996 and 0.9997, respectively. The limits of detection (LODs) were 0.003, 0.001 and 0.002 μg/kg for Rg1, Re and Rb1, respectively. The recoveries were 98.3%~101.3% with the relative standard deviations (RSDs) of 0.8%, 1.1% and 1.5% (n=6). Conclusions The established method has the advantages of rapid determination, low column pressure and less reagents consumption, which is suitable for the detection of ginsenosides in Panax ginsen.
标题：新型C18核壳结构色谱柱快速分析人参中的 3种皂苷
英文标题：Rapid determination of 3 kinds of ginsenosides in Panax ginseng by new C18 core-shell column
作者：
岳焕新 浙江师范大学物理化学研究所
薛昆鹏 浙江师范大学物理化学研究所；浙江月旭材料科技有限公司
金小青 浙江师范大学物理化学研究所；浙江月旭材料科技有限公司
李良翔 浙江月旭材料科技有限公司
赵岳星 浙江师范大学物理化学研究所；浙江月旭材料科技有限公司
中文关键词:人参,人参皂苷,高效液相色谱,核壳色谱柱,
英文关键词:Panax ginseng,ginsenoside,high performance liquid chromatography,core-shell column,
发表日期：2016-05-25
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