Objective Account of the increasing amount of transgenic soybean and soybean products, the safety problem has received more and more attention from consumers. In order to satisfy consumers’ right of informed choice, a rapid method of detecting genetically modified products was established in this study. Methods CaMV35S promoter and NOS terminator in soybean products were taken as detection target. The double PCR reaction system and conditions were optimized, including primer ratio and enzyme amount. Agarose gel and polyacrylamide gel electrophoresis were applied to analyze PCR products, and the silver staining conditions for polyacrylamide gel electrophoresis were explored. Results The polyacrylamide gel electrophoresis was established and could distinguish CaMV35S promoter and NOS terminator better than agarose gel. Conclusion The results showed that double PCR and polyacrylamide gel electrophoresis in this experiment were suitable for the analytic detection of two foreign genes in transgenic soybean products, and it could be a guidance for detecting transgenic food.
标题：豆制品中转基因成分的二重PCR检测体系的优化研究
英文标题：Optimizing of double PCR system for detecting transgenic ingredients in soybean products
