衍生化法测定山药中还原型谷胱甘肽GSH和总巯基-SH含量
目的 用衍生化法测定山药中还原型谷胱甘肽(GSH)和总巯基(-SH)的含量。方法 利用4,4?-二硫代联吡啶(4-DPS)和邻苯二甲醛(OPA)为衍生化试剂,采用细胞破壁和衍生化为一体的样品前处理方法, 分别用UV-vis分光光度法和荧光光谱法测定了山药鲜品中总巯基(-SH)、GSH的含量。结果 山药中含较丰富的总巯基和GSH, 含量分别达0.4556 ?mol/g和0.1792 ?mol/g。UV-vis分光光度法和荧光光谱法测定方法的检测限分别为1.0458 ?mol/L和0.5666 ?mol/L, 回收率分别为98.73%和98.40%。结论 本研究对山药中巯基化合物的含量、测定方法学和样品前处理方法提供了重要的基础数据和参考依据。
Objective To detect reduced glutathione (GSH) and total thiols (-SH) in Rhizoma Dioscorea by derivatization methods. Methods Total thiols and reduced glutathione (GSH) in Rhizoma Dioscorea were determined by UV-vis spectrophotometry and fluorophotometry using 4,4?-dithiodipyridine (4-DPS) and O-phthaldehyde (OPA) as derivatization agents. Samples were prepared by simultaneous derivatization during cell rupture, Results 0.4556 μmol/g of total thiols and 0.1792 μmol/g of GSH were detected in the samples . The detection limits for UV-vis spectrophotometry and fluorophotometry were 1.0458 μmol/L and 0.5666 μmol/L, and recoveries were 98.73% and 98.40%, respectively. Conclusion This study provides useful data and references for the content of thiol compounds in Rhizoma Dioscore determination methodology and pretreatment methods for plant sample analysis.
标题：衍生化法测定山药中还原型谷胱甘肽(GSH)和总巯基(-SH)含量
英文标题：Determination of reduced glutathione (GSH) and total thiols (-SH) in Rhizoma Dioscorea by derivatization methods
作者：
金京实 延边大学长白山生物资源与功能分子教育部重点实验室
沈丽 延边大学长白山生物资源与功能分子教育部重点实验室
崔胜云 延边大学长白山生物资源与功能分子教育部重点实验室
中文关键词:山药,还原型谷胱甘肽,4,4-二硫代联吡啶,邻苯二甲醛,衍生化,
英文关键词:Rhizoma Dioscore,reduced glutathione,4,4-dithiodipyridine,O-phthaldehyde,derivatization,
发表日期：2013-06-14
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