环介导等温扩增法检测食品过敏原大豆成分
目的 建立食品过敏原大豆成分的环介导等温扩增(LAMP)分析方法。方法 根据大豆的内源管家基因Lectin基因设计6条过敏原大豆的特异性引物(两条内引物, 两条外引物和两条环引物), 建立LAMP检测方法。结果 环介导等温扩增法在63 ℃条件下, 40 min内可检出过敏原大豆成分, 该方法能够有效对大豆成分进行快速检测, 具有较强的特异性, 灵敏度可达0.01%(w/w)大豆粉。结论 该方法特异性强, 灵敏度高, 可以快速、准确检测食品中过敏原大豆成分。
Objective To establish a detection method of allergen soybean by loop-mediated isothermal amplification assay (LAMP). Methods The method of LAMP was established for the detection of soybean in food. Results The soybean could be rapidly and specifically detected by LAMP method within 40 min at constant temperature of 63 ℃. The method was specific for soybean, and the limit of detection was determined as 0.01% (w/w) soybean powder. Conclusion The established LAMP method is effective, specific and sensitive for the detection of soybean, and it is suitable for the detection of soybean in food.
标题：环介导等温扩增法检测食品过敏原大豆成分
英文标题：Detection of allergen soybean by loop-mediated
作者：
张舒亚 上海出入境检验检疫局
李富威 上海海洋大学食品学院
于翠 上海出入境检验检疫局
宋青 上海出入境检验检疫局
张坤 上海出入境检验检疫局
中文关键词:食品过敏原,大豆,LAMP,检测,
英文关键词:food allergen,soybean,loop-mediated isothermal amplification,detection,
发表日期：2012-11-26
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