Objective To establish the real-time fluorescence RT-PCR detection method of norovirus GI and GII in frozen strawberry, and to apply to actual sample testing. Methods After pretreation of strawberry samples, concentrating of viruses, extracting and purifying of virus RNA, the samples were determinated by real-time fluorescence RT-PCR. Results The nucleic acid extraction method could effectively remove the inhibitory factors. According to the test of 104 inspection samples, the results were all negative. Conclusion The established system of nucleic acid extraction and real-time fluorescence RT-PCR detection method is suit-able for the detection of norovirus GI and GII in frozen strawberry samples.
标题：实时荧光RT-PCR检测冷冻草莓中诺如病毒
英文标题：Detection of norovirus in frozen strawberry by real-time fluorescent RT-PCR
