基于柱切换技术的高压液相色谱法检测奶粉中的维生素D
目的 建立奶粉中维生素D的柱切换式高压液相色谱分析方法。方法 奶粉样品经过皂化处理、石油醚提取、脱水浓缩后, 用柱切换式高压液相色谱仪进行检测。结果 维生素D3在0.2~1.0 ?g/mL范围内线性关系良好, 回归方程Y=1.74181e?006X?0.00662911, 相关系数r=0.9956, 检出限为0.01 ?g/mL, 定量限为0.2 mg/kg, RSD为2.12%, 平均回收率为 96.8%。结论 该方法简单、快速、准确, 为复杂基质食品中低含量物质的检测提供一定参考。
Objective To establish a method for the determination of vitamin D in milk powder by high pressure chromatography based on column-switching methods. Methods After saponification treatment, milk powder was extracted with petroleum ether. The extract was dehydrated with anhydrous sodium sulfate, concentrated, and analyzed by HPLC. Results The method showed a good linearity over the range of 0.2~1 μg/mL for vitamin D with r=0.9956. The linear equations was Y =1.74181e-006X-0.00662911 and the average recoveries were 96.8% with the relative standard deviations(RSDs)of 2.12%. The LOD and LOQ were 0.01 μg/mL and 0.2 mg/kg, respectively. Conclusion The method is simply, rapid and accurate, and it provides a reference for the detection of trace substance in complex matrix.
标题：基于柱切换技术的高压液相色谱法检测奶粉中的维生素D
英文标题：Detection of vitamin D in milk powder by HPLC based on column-switching system
作者：
周提军 资生堂中国投资有限公司先端科学事业推进部应用技术中心
王莎 资生堂中国投资有限公司先端科学事业推进部应用技术中心
李兵 资生堂中国投资有限公司先端科学事业推进部应用技术中心
荒井裕子 资生堂中国投资有限公司先端科学事业推进部应用技术中心
城田修 资生堂中国投资有限公司先端科学事业推进部应用技术中心
中文关键词:阀切换,维生素D,高压液相色谱法,
英文关键词:column-switching,vitamin D,high pressure chromatography (HPLC),
发表日期：2012-11-09
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