敞开式离子化质谱新技术及其应用
敞开式离子化质谱(Ambient Mass Spectrometry, AMS)是近年来新兴的一种无需或者只需很少样品前处理步骤, 在敞开的大气压环境中可直接对物体表面物质进行离子化的质谱分析技术。本实验室基于敞开式离子源实时直接分析(Direct Analysis in Real Time, DART), 进行了多方面的研究。主要包括三个方面：第一, 应用研究, 将简单的样品前处理技术和DART-MS结合, 建立了有针对性的快速检测方法; 第二, 实现正相液相色谱(Normal Phase Liquid Chromatography, NPLC)和DART-MS的在线联用, 解决了NPLC-MS联用中正相色谱流动相不利于样品离子化的问题, 并利用该方法对NPLC分离的手性化合物进行了有效检测; 第三, 构建了一套新的AMS装置—等离子体辅助多波长激光解吸离子化质谱(Plasma Assisted Multiwavelength Laser Desorption Ionization Mass Spectrometry, PAMLDI-MS)。该装置将激光解吸附和等离子体辅助离子化技术相组合, 并且将其与简单的TLC快速分离结合, 对一些小分子混合物进行了分离检测; 实验室还借助简单的铅笔芯中石墨涂覆的滤纸作为PAMLDI-MS的进样基底, 发现石墨涂覆的滤纸对于待测样品有很好的基质辅助信号增强作用。
Ambient mass spectrometry (AMS) is a kind of newly emerged technology performed under ambient conditions that allows the direct analysis of sample or sample surfaces with little or no sample pretreatment. Based on direct analysis in real time mass spectrometry (DART-MS), our laboratory investigated some new techniques for ionization of ambient mass spectrometry and explored their applications. First, rapid targeted screening methods were developed through combination of easy sample pre-treatment with DART-MS. Second, online coupling of normal phase liquid chromatography (NPLC) and MS was realized for chiral separation, greatly improving the ionization efficiency of the analytes in the normal phase mobile phase. For new ambient ionization source research, our laboratory fabricated plasma assisted multiwavelength laser desorption ionization mass spectrometry (PAMLDI-MS) system, which combined laser desorption and plasma-assisted ionization together. This system has been applied in the analysis of low molecular weight compounds separated on thin layer chromatography (TLC). In addition, a piece of graphite-coated paper was utilized for the sample introduction, which greatly improved the detection sensitivity of analytes in the above system.
标题：敞开式离子化质谱新技术及其应用
英文标题：New techniques of ambient mass spectrometry and their applications
作者：
栗则 北京大学化学与分子工程学院
张佳玲 北京大学化学与分子工程学院
常翠兰 北京大学化学与分子工程学院
白玉 北京大学化学与分子工程学院
刘虎威 北京大学化学与分子工程学院
中文关键词:敞开式离子化质谱,实时直接分析,接口,离子源,基质,
英文关键词:ambient mass spectrometry(AMS),direct analysis in real time(DART),interface,ionization source,matrix,
发表日期：2012-10-11
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