紫外光度法测定蜂蜜中残留土霉素和盐酸金霉素的含量
目的 建立测定蜂蜜中土霉素和盐酸金霉素含量的方法。方法 采用紫外光度法测定土霉素和盐酸金霉素的含量。结果 在甲醇溶液中, 土霉素和盐酸金霉素的最大吸收波长分别在364 nm和319 nm处, 在0.56~6.72 ?g/mL和1.03~20.60 ?g/mL浓度范围内有很好的线性关系, 回归方程和相关系数分别为Y= 0.0254X?0.00644, r=0.99355和Y=0.02391 0.02756X, r=0.99493。结论 该方法操作简单, 重现性好, 可准确用于蜂蜜样品中土霉素和盐酸金霉素含量的测定, 结果满意。
Objective To establish a method for determining the content of oxytetracycline and chlortetracycline hydrochloride of honey samples. Methods The content of oxytetracycline and the chlortetracycline hydrochloride were absorbed by ultraviolet (UV) spectrophotometry. Results In methanol solution, the maximum absorption wavelengths of oxytetracycline and the chlortetracycline hydrochloride were at 364 nm and 319 nm, respectively. It had a good linear relationship between the range of 0.56~6.72 ug/mL and 1.03~20.60 ug/mL concentration, and the regression equation and the correlation coefficient were Y=0.0254X? 0.00644, r=0.99355 and Y=0.02391 0.02756X, r=0.99493. Conclusion The method has the advantages of simple operation, good reproducibility, and accuracy, and it can be used to determine the content of oxytetracycline and chlortetracycline hydrochloride of honey samples.
标题：紫外光度法测定蜂蜜中残留土霉素和盐酸金霉素的含量
英文标题：Determination of oxytetracycline and chlortetracycline hydrochloride in honey by ultraviolet spectrophotometry
作者：
阿布都拉.艾尼瓦尔 新疆农业大学化学工程学院
加列西 新疆农业大学化学工程学院
阿布都瓦依提.玉苏甫 新疆农业大学化学工程学院
中文关键词:蜂蜜,抗生素,药物残留,紫外光度法,
英文关键词:honey,antibiotic,drug residue,ultraviolet spectrophotometry,
发表日期：2012-05-01
- 文件大小:
- 393.16 KB
- 下载次数:
- 60
-
高速下载
|
|
