Chain replacement amplification primers designed according to the ompW genetic fragments sequence (strand displacement amplification). SDA fast detection method of Vibrio cholerae was established. The sensitivity and specificity of the designed primers were studied. The detection sensitivity of Vibrio cholerae reached 7.0×102 cfu/mL. SDA technology established can be specially amplifying Vibrio cholerae.
标题:霍乱弧菌SDA-琼脂糖凝胶电泳检测技术研究
英文标题:Detection technique of Vibrio cholerae by strand displacement amplification