基于多孔碳球修饰硼掺杂金刚石电极的 甲基对硫磷检测
目的 研究甲基对硫磷检测的快速检测方法。方法 用多孔碳球材料修饰硼掺杂金刚石(BDD)电极, 将乙酰胆碱酯酶(AChE)固定在修饰电极表面制得酶传感器, 用于甲基对硫磷的检测。对所制备的酶传感器进行电化学表征, 对实验参数进行优化。结果 底物氯化乙酰胆碱的产物在AChE/BDD电极和AChE/多孔碳球/BDD电极上的氧化峰电流分别为0.4930 μA和1.102 μA, 峰电流提高了123.53%。在AChE/多孔碳球/BDD电极上, 建立抑制率与甲基对硫磷浓度的负对数的对应关系, 在浓度范围10?10~10-7g/L内呈良好的线性关系, 线性方程为Y=?13.20297X+148.375, 相关系数R2为0.9985。按抑制率为10%计算检出限为3.02×10?12 g/L。对实际样品黄瓜汁进行检测, 样品回收率为92.60%~102.00%。结论 此方法简单、快速、灵敏度高, 适合用于实际样品中的甲基对硫磷残留分析。
Objective To study the rapid determination method of methyl parathion. Methods Porous carbon spheres were prepared and used to modify boron-doped diamond (BDD) electrode. The acetylcholinesterase (AChE) was fixed to the modified electrode surface, and then was used to detect methyl parathion. The ob-tained enzyme sensor was characterized electrochemically, and the experimental parameters were optimized. Results The oxidation peak current of ATCl product was 0.4930 μA and 1.102 μA on the AChE/BDD electrode and AChE/porous carbon spheres/BDD electrode, and the peak current was enhanced by 123.53%. The sensor had a good linear relationship with the concentration of methyl parathion in the range of 10-10~10-7g/L, with a linear equation of Y=-13.20297X+148.375 (R2=0.9985) and a detection limit of 3.02×10-12 g/L. The sensor could be used for detecting cucumber juice samples, with recovery rate of 92.60%~102.00%. Conclusion This method is simple, fast, and highly sensitive, and it is suitable for the detection of methyl parathion.
标题：基于多孔碳球修饰硼掺杂金刚石电极的 甲基对硫磷检测
英文标题：Determination of methyl parathion based on porous carbon spheres-modified boron-doped diamond electrode
作者：
赵凤娟 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
罗耀 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
聂冬锐 辽宁出入境检验检疫局技术中心
沈金灿 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
熊贝贝 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
韩瑞阳 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
岳振峰 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
中文关键词:多孔碳球,BDD电极,乙酰胆碱酯酶,氯化乙酰胆碱,甲基对硫磷,
英文关键词:porous carbon spheres,boron-doped diamond electrode,acetylcholinesterase,acetylcholine chloride,methyl parathion,
发表日期：2014-10-09
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