转基因羊奶表达重组人溶菌酶的急性毒性及 过敏性研究
目的 研究该转基因羊奶表达的重组人溶菌酶的急性毒性和过敏性, 确保食品质量安全。方法 通过小鼠经口急性毒性、体外模拟胃肠道消化和体外热稳定性试验进行研究。结果 小鼠经口急性毒性试验结果显示, 重组人溶菌酶对小鼠最大耐受剂量＞5000 mg/kg.BW, 属实际无毒。体外模拟胃肠道消化试验结果显示, 重组人溶菌酶在模拟胃液中2~30 min内全部消化; 在模拟肠液中15 s~2 min内全部消化; 体外热稳定性试验结果显示, 重组人溶菌酶在100℃加热60 min不会降解。结论 该重组人溶菌酶的毒性与过敏性风险较低, 可以安全应用于食品行业。
Objective To ensure the food safety, this study was focused on the acute toxicity test and allergenicity assessment of lysozyme expressed in milk from transgenic goats andallergenicity assessment in vitro. Methods Oral acute toxicity test, thermal stability experiment and simulated gastrointestinal digestion experiment were put out to estimate the food safety of lysozyme. Results The oral acute toxicity test indicated the lysozyme protein caused no adverse effects in mice by intragastric infusion at a high dose of 5 g/kg.BW. The results of simulated gastrointestinal digestion experiment indicate that lysozyme was digested within 2~30 min in stimulated gastric fluid, and within 15 s~2 min in stimulated intestinal fluid. The thermal stability experiment showed that the lysozyme had stability after heating 60 min at 100℃. Conclusion The food safety evaluation of the lysozyme showed that it had littlerisk on allergenicity and toxicity, and it could be used in food industry safely.
标题：转基因羊奶表达重组人溶菌酶的急性毒性及 过敏性研究
英文标题：Study on toxicity and allergy of lysozyme expressed in milk from transgenic goats
作者：
吕超 中国农业大学食品科学与营养工程学院
包琪 中国农业大学食品科学与营养工程学院
邹世颖 中国农业大学食品科学与营养工程学院;农业部转基因生物食用安全监督检验测试中心(北京)
陈思媛 中国农业大学食品科学与营养工程学院
贺晓云 中国农业大学食品科学与营养工程学院;农业部转基因生物食用安全监督检验测试中心(北京)
黄昆仑 中国农业大学食品科学与营养工程学院;农业部转基因生物食用安全监督检验测试中心(北京)
中文关键词:人溶菌酶,急性毒性,致敏性,模拟消化,
英文关键词:human lysozyme,acute toxicity,allergenicity,simulated digestion,
发表日期：2014-07-24
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