间接酶联免疫法检测鲑鳟鱼类传染性 造血器官坏死病病毒
目的 建立快速检测传染性造血器官坏死病病毒(IHNV)的间接酶联免疫法。方法 以传染性造血器官坏死病毒G蛋白(IHNV-G)为抗原, 免疫实验白兔, 制备兔抗IHNV免疫血清, 通过ELISA对兔抗血清进行效价及特异性评估, 并通过PCR方法进行验证。结果 应用间接ELISA 技术, 检测到抗血清对IHNV-G和IHNV的效价分别达到1:32000和1:16000, 与其他对照病毒无交叉反应。与PCR检测结果符合率达到98%。结论 本文建立的间接酶联免疫法方法具有良好的灵敏性和特异性, 为IHNV的检测提供了快捷、高效的技术手段。
Objective To establish an indirect enzyme-linked immune assays (ELISA) for the identification of infectious hematopoietic necrosis virus (IHNV). Methods An experimental rabbit was immunized to pro-duce anti-IHNV serum with IHNV-G protein. To assess the titer and specificity of the anti-serum, the ELISA test was used. Further, PCR was used to verify the above ELISA experimental result. Results The titer of anti-serum of IHNV-G and IHNV reached to 1:32000 and 1:16000, respectively, moreover, the cross-reaction with other virus was 0%, and ELISA results was with 98% accordance rate with the method of PCR. Conclusion The indirect-ELISA of this research has a good specificity and sensitivity, and it is suitable for rapid detection and epidemiological investigation of IHNV infection in trout.
标题：间接酶联免疫法检测鲑鳟鱼类传染性 造血器官坏死病病毒
英文标题：Identification of infectious hematopoietic necrosis virus in trout by indirect-enzyme-linked immune assays
作者：
金爽 辽宁大学生命科学院
金莉莉 辽宁大学生命科学院
郭欣硕 辽宁省水产品质量安全检验检测局
蔺翠翠 辽宁省水产品质量安全检验检测局
罗靳 辽宁省水产品质量安全检验检测局
王秋雨 辽宁大学生命科学院
中文关键词:鲑鳟鱼,传染性造血器官坏死病病毒,病毒糖蛋白,间接酶联免疫法,
英文关键词:trout,infectious hematopoietic necrosis virus,IHNV-G protein,indirect-enzyme-linked immune assays,
发表日期：2014-07-24
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