Objective Comparison the methods of amino acid analyzer (AAA) and ultra-performance liquid chromatography (UPLC) for determination of hydrolysis amino acids in human milk. Methods Hydrolysis amino acids were analysed with C18 column and AQC precolumn derivation by UPLC method; and ion ex-change chromatography column and ninhydrinpostcolumn derivation by AAA method. Results Two methods provided similar validation results. The difference of analysis result using two methods was below 4.65%. Conclusion Both methods can be used for the quantitation of hydrolysis amino acids.
标题:液相色谱法与氨基酸分析仪法测定人乳中 水解氨基酸的比较研究
英文标题:Comparative study on quantification of hydrolysis amino acids in human milk using liquid chromatography and amino acid analyzer