响应面法优化苦荞壳中总黄酮的提取工艺
目的 优化苦荞壳中总黄酮提取工艺。方法 用浸提法、恒温振荡提取法、索氏提取法、高压提取法、超声波提取法进行实验, 以最大提取率及总黄酮含量为指标进行对比, 优选出浸提法为最佳提取方法。采用响应面分析法对影响苦荞壳中总黄酮提取率的4个主要因素, 乙醇浓度、提取温度、提取时间、料液比进行研究, 并以Box-Behnken中心组合设计建立数学模型。结果 浸提法最佳提取工艺为乙醇浓度45%、提取温度85 ℃、提取时间2.5 h、料液比1:40, 此条件下总黄酮含量为15.7 mg/g, 提取率为1.57%, 回收率为98.2%, 回归模型的预测值(16.4 mg/g)与实测值的相对误差为4.6%。结论 以Box-Behnken设计结合响应面分析法能较好地预测苦荞壳中总黄酮的含量, 该方法简便省时, 是苦荞壳中提取总黄酮的有效途径。
Objective To optimize the extraction technology of total flavonoids from tartary buckwheat shell. Methods The maximum extraction rate and content of total flavonoids were compared by soak extrac-tion method, constant temperature oscillation extraction method, soxhlet extraction method, high-pressure extraction method, and ultrasonic wave extraction method. Soak extraction method was selected as the optimization extraction method. Effects of ethanol concentration, extraction temperature, extraction time and liquid to solid ratio on total flavonoids extraction rate were studied using response surface methodology, and the experiment mathematical model was established according to Box-Behnken central composite experimental design. Results The optimum extraction conditions are ethanol concentration 45%, extraction temperature85℃, extraction time 2.5 h, and the liquid to solid ratio 1:40. Under the optimum conditions, the flavonoids yield is 15.7 mg/g, the maximum extraction rate is 1.57%, close to predictive value16.4 mg/g. Conclusion The combination of Box-Behnken design and response surface analysis can well optimize the extraction technology of total flavonoids from tartary buckwheat shell.
标题：响应面法优化苦荞壳中总黄酮的提取工艺
英文标题：Optimization of technology for extracting total flavonoids from tartary buckwheat shell by response surface methodology
作者：
王廷璞 天水师范学院生命科学与化学学院
赵强 天水师范学院生命科学与化学学院；中国科学院西北高原生物研究所
袁建军 天水师范学院生命科学与化学学院
中文关键词:苦荞壳,总黄酮,响应面,
英文关键词:tartary buckwheat shell,total flavonoids,response surface methodology,
发表日期：2014-05-19
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