多功能柱净化-高效液相色谱法检测粮谷 及其制品中的呕吐毒素
目的 建立呕吐毒素的多功能柱净化-高效液相色谱检测方法, 并建立液相色谱-串联质谱确证方法。方法 样品经乙腈-水溶液(84﹕16, v:v)提取、多功能柱净化、吹干、定容后, 最后以液相色谱-紫外检测器进行定量分析, 阳性检出以液相色谱-串联质谱法确证。结果 该方法定量限为0.3 μg/g, 在0.5、1.0、1.5 μg/g的添加水平下, 样品回收率为66.0%~98.3%, 室内相对标准偏差(RSDr)为1.9 %~12.7 %, 室内HorRat值为0.1~0.7。结论 该方法操作简便, 灵敏度高, 选择性好, 适用于粮谷及其制品中呕吐毒素的检测。
Objectives To establish a simple and rapid determination method of deoxynivalenol (DON) in grain and grain products by multifunctional column clean-up and high performance liquid chromatography (HPLC). Methods Samples were extracted with acetonitrile-water (84﹕16, v:v), and the extracted liquid was purified by multifunctional columns. After drying, and re-dissolved in the mobile phase, the toxins were subjected to HPLC-UV analysis. The positive results were confirmed using high performance liquid chromatogra-phy-tandem mass spectrometry (HPLC-MS/MS) analysis. Results Recoveries of DON added at levels rang-ing from 0.5 to 1.5 μg/g for all tested samples were from 66.0% to 98.3%. Relative standard deviation (RSDr) ranged from 1.9% to 12.7%. Within-laboratory HorRat values were from 0.1 to 0.7. Conclusion This method is simply clean-up, rapid and of high sensitivity, and suitable for the identification and quantification of DON in grain and grain products.
标题：多功能柱净化-高效液相色谱法检测粮谷 及其制品中的呕吐毒素
英文标题：Determination of deoxynivalenol in grain and grain products by multifunctional column clean-up and high performance liquid chromatography
作者：
鲍蕾 山东出入境检验检疫局技术中心
吴振兴 山东出入境检验检疫局技术中心
石媛媛 山东出入境检验检疫局技术中心
静平 山东出入境检验检疫局技术中心
梁成珠 山东出入境检验检疫局技术中心
中文关键词:呕吐毒素,多功能柱,液相色谱,粮谷及其制品,液相色谱-串联质谱,
英文关键词:DON,multifunctional column,High Performance Liquid Chromatography,Grain and Grain Products,HPLC/MS/MS,
发表日期：2014-03-17
- 文件大小:
- 1.19 MB
- 下载次数:
- 60
-
高速下载
|
|
