两株耐铬菌的分离鉴定及对CrⅥ污染土壤的治理

文档摘要：为实现高质量比铬污染场地的微生物修复,从华北某废弃铬盐厂取土样分离出两株高效铬还原菌,并对两株菌进行16SrRNA鉴定,分别命名为Agrobacteriumsp.Cr-1(Cr-1)和Lysinibacillussp.Cr-2(Cr-2).将两菌株分别投入高质量比铬污染土壤中,通过分析土壤Cr(Ⅵ)质量比、pH值、铬形态的变化,探究两菌株对高质量比Cr(Ⅵ)污染土壤的治理能力.通过细菌多样性和代谢组学分析,根据细菌种类、代谢物和代谢物通路的差异分析了两菌株的Cr(Ⅵ)解毒及还原的原理.当反应42d后,投加Agrobacteriumsp.Cr-1和Lysinibacillussp.Cr-2的土壤Cr(Ⅵ)质量比分别由1100mg/kg降至33.49mg/kg和92.29mg/kg.研究显示:两种菌株均能够实现对高质量比Cr(Ⅵ)污染土壤的高效修复.

Abstract：Toachievehigh-qualitymicrobialremediationofchromium-contaminatedsites,twostrainsofhighlychromium-resistantbacteriawereisolatedfromsoilsamplesnearachromateplantinNorthChina.Basedonthecomparisonof16SrRNAsequencesinGenBankatNCBI,strainsCr-1andCr-2showed＞99％homologywithAgrobacteriumtumefaciensandLysinibacillusfusiformis,respectively.ThetwostrainswerenamedasAgrobacteriumsp.Cr-1andLysinibacillussp.Cr-2,respectively.7daysafterinoculationoftwostrains,themassratioofsoilCr(Ⅵ)decreasedrapidly,andtheCr(Ⅵ)removalratesofAgrobacteriumsp.Cr-1andLysinibacillussp.Cr-2was94.48％and85.12％,respectively.After42days'operation,themassratioofCr(Ⅵ)insoilcontainingAgrobacteriumsp.Cr-1decreasedfrom1100mg/kgto33.49mg/kg,whilethatofLysinibacillussp.Cr-2wasreducedto92.29mg/kg.TheeffectivereductionofhighmassratioCr(Ⅵ)indicatedtwostrainsexhibitedstrongresistancetoCr(Ⅵ)andhighreductioncapability.AnalysisoftheCrformsinsoilrevealedagradualtransitionofCrtoFe-Mnboundandresidualstateswithlowtoxicityandbioavailability,resultinginagreatreductionofitsmigrationandbiotoxicity.Bacterialdiversityandmetabolomicsanalysisofthesoilafter42daysofreactionshowedthattheadditionofbothstrainsnotonlycoulddirectlyparticipateintheCr(Ⅵ)-reductionpathwaybutalsopromotedthegrowthandreproductionofchromium-reduction-relatedbacteriainthesoil.Analysisofdifferentialmetabolitesandmetabolicpathwaysshowedthatbothstrainsreducedthedamageofchromiumtoreducingbacteriabysecretingorganicacidsandotherrelatedsubstances.ThereductionofCr(Ⅵ)wasachievedthroughthetransferofCrfromextracellulartoinsidethroughABCtransportersandmetabolitesrelatedtosubstancetransport,suchasD-galactosamine,cis-4-hydroxy-D-proline,andL-pipecolicacid.Thisstudyshowsthatbothstrainscanachieveefficientmicrobialremediationofhighmassratiohexavalentchromium-contaminatedsoil.

作者：吴卿  李倩男  张颖  刘睿怡  彭森  万芮含Author：WUQing  LIQiannan  ZHANGYing  LIURuiyi  PENGSen  WANRuihan
作者单位：天津大学环境科学与工程学院,天津300350
刊名：安全与环境学报 ISTICPKU
Journal：JournalofSafetyandEnvironment
年，卷(期)：2024, 24(6)
分类号：X53
关键词：环境工程学  Cr(Ⅵ)污染土壤  生物还原  细菌多样性  代谢组学  
Keywords：environmentalengineering  Cr(Ⅵ)contaminatedsoil  bio-reduction  bacterialdiversity  metabolomics  
机标分类号：S852.65X172S181.3
在线出版日期：2024年7月4日
基金项目：两株耐铬菌的分离鉴定及对Cr(Ⅵ)污染土壤的治理[
期刊论文]  安全与环境学报--2024, 24(6)吴卿  李倩男  张颖  刘睿怡  彭森  万芮含为实现高质量比铬污染场地的微生物修复,从华北某废弃铬盐厂取土样分离出两株高效铬还原菌,并对两株菌进行16SrRNA鉴定,分别命名为Agrobacteriumsp.Cr-1(Cr-1)和Lysinibacillussp.Cr-2(Cr-2).将两菌株分别投入高质量...参考文献和引证文献
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关键词：环境工程学,Cr(Ⅵ)污染土壤,生物还原,细菌多样性,代谢组学,

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